Calcium ions inhibited streaming in detached leaves, whereas magnesium ions. Cytoplasmic streaming in elodea canadian researchgate. Cyclosis or cytoplasmic streaming can be easily seen in cells of canadian pondweed. Nuclear dna content and number of chloroplasts in elodea. Cytoplasmic streaming, or cyclosis, is an energyconsuming event that occurs in plant.
Although its not an organelle, it is common to see cytoplasmic streaming in elodea cells. About 1015 minutes after preparing a wet mount of an elodea leaf, evaporation of some of the water makes it possible to see chloroplasts in motion inside of the cytoplasm. Observations were made on detailed leaves of elodea and streaming was observed in most leaf cells, velocities from 0. Download citation cytoplasmic streaming in elodea canadian cytoplasmic streaming in the. In conclusion, it may be safely concluded, that the supply of rna, which is dependent on nuclear function, is of paramount importance in the control of the. The role of cyclosis in chloroplast functioning should be most conspicuous under conditions mimicking natural mosaic illumination and consequent alternation of cell regions with dominant dark and. This plant may be known by one or more common names in different places, and some are listed above.
Cytoplasmic streaming in plant cells elodea dic 1250x. Cytoplasmic streaming facillitates the movement of materials within the cell and plays a role in the movement of substances from cell to cell. Estimate the size of an individual cell and the size of a chloroplast. Cytoplasmic streaming an overview sciencedirect topics. Using elodea as a live model of the eukaryotic cell nsta news. For my ib biology class i have to create a lab with the overall prompt of cytoplasmic streaming in elodea plants. Observing chloroplasts in movement in an elodea cell is like watching a busy, bustling mass of pedestrians from a building high above. Cytoplasmic streaming in plant cells emerges naturally by. This is a recording on 400x using a light microscope. As the slide warms from the light of the microscope, you may see the chloroplasts moving, a process called cytoplasmic streaming.
Using leaf cells of elodea densa, investigations were made on the role of nucleus in some activities of protoplasm, by comparing the behaviours of nucleated and enucleated halves of protoplast, which were separated by means of plasmolytic treatment of intact cells plasmolytic treatment caused a remarkable chloroplastsystrophe, which was more pronounced in. Start studying structure and function of living cells. Elodea canadensis canadian waterweed this plant can be weedy or invasive according to the authoritative sources noted below. Get a wet mount slide of an elodea leaf from your teacher. Any of various small, submersed plants of the genus elodea, having narrow, whorled or opposite leaves and small axillary flowers. It has been quite some time since my high school biology class so when i saw that we would be studying elodea cells in lab this week i had a trip down memory lane to my first encounter with the plant. Elodea is a water plant native to canada, often used in aquariums. Cytoplasmic streaming, the movement of the fluid substance cytoplasm within a plant or animal cell. The chloroplasts can be seen moving around the cell. Many cells exhibit largescale active circulation of their entire fluid contents, a process termed cytoplasmic streaming. Effect of cytoplasmic streaming on photosynthetic activity.
If not, you can purchase it from a school supplier. It shows an elodea leaf undergoing cytoplasmic streaming aka cyclosis. It is a monocotyledon from the hydrocharitaceae family. Experiments to determine the early labeled photosynthetic products in elodea canadensis show that after 2 seconds of exposure to nah 14 co 3, 45% of the 14 c incorporated is located in malate and aspartate. Elodea pondweed experiments on microscopes 4 schools. The most disordered instances often function as part of cellular patterning processes, as in drosophila. Describe the locations of the nucleus and the majority of the chloro. At 400x magnification, it was truly amazing to see the chloroplasts moving in the cells.
Cytoplasmic streaming, also called protoplasmic streaming and cyclosis, is where there is flow. Answer to describe the functions of the components in elodea. Plants profile for elodea canadensis canadian waterweed. Observe the leaf at 40x and carefully draw what you see in the space marked. Instead of just reading about cytoplasmic streaming in a textbook, we got. They jostle and slide and scoot around the cell, often sticking near the edges of the cell but sometimes seeming to fill the cell entirely with constant motion. Elodea is commonly used in undergraduate teaching to show cytoplasmic streaming and osmolarity. Also, if the light is closer, the process will be faster.
Photosynthesis in elodea lab essay example graduateway. Surrounding the cell is the cell wall, which appears as relatively thick boundaries between the elodea cells. A physical perspective on cytoplasmic streaming interface focus. Classified in the frogsbit family hydrocharitaceae, elodea is native to the americas and is also widely used as aquarium vegetation and laboratory demonstrations of cellular activities. Homeostasis involves the maintenance of a stable set of conditions inside an organisms cells. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
The movement of particles throughout the cells cytoplasm is facilitated by the cytoskeleton. Cytoplasmic streaming, the movement of the cytoplasm and inclusions in it. The effect of environmental stimuli on cytoplasmic viscosity. Structure and function of living cells flashcards quizlet. Chloroplasts in the elodea cell are throughout the cytoplasm and continually move around, but mostly cling to the cell wall. Elodea is a submerged, freshwater perennial, generally rooted on the bottom in depths of up to 20 feet or drifting. The nucleus of elodea is usually found somewhere in the perimeter of each cell. Just inside the cell wall, you may or may not be able to see the cell membrane. Phosphoglyceric acid and sugars account for 27% of the label during similar exposures. I was thinking i could figure out if the decrease in sunlight decreases the speed of cytoplasmic streaming, however i am not sure how to measure the speed of cytoplasmic streaming. Elodea elodea canadensis is a common plant that grows in fresh water ponds, lakes, and is often used in freshwater aquariums. This lab was meant to show us the chloroplasts moving around cells in the cytoplasm.
The procedure for this would be to leave the elodea plant in water with sodium bicarbonate baking soda increases carbon dioxide in water, set up the lamp at 5cm distance and start the timer for 3 minutes. The flow is driven by forces from the cytoskeleton, and it is likely that the function of flow is at least partly to speed up transport of molecules and organelles around the cell. Elodea, genus of five or six species of submerged aquatic plants in the frogsbit family hydrocharitaceae, useful in aquariums and in laboratory demonstrations of cellular activities. Vigorous streaming of the cytoplasmic matrix is a basic feature of intracellular movement, and the mechanisms and significance of cytoplasmic streaming of multiple modes have long been attractive subjects for many researchers shimmen, 2007. Cytoplasmic streaming plays an important role in cell processes since it promotes solute exchange between the cytoplasm and organelles and enables lateral transport for extensive distances. The function of cytoplasm is also a means of transport for genetic material and the products of cellular respiration. Elodea is a genus of submerged aquatic plants used in aquariums. Nuclear control of chloroplast activity in elodea leaf cells.
Last week, we looked at cytoplasmic streaming of elodea leaf cells in class. What is the function of the chloroplasts in elodea cells. Nuclear dna content, chloroplast number, growth regulators. Virgin 1954 observed that the chloroplasts in the leaf cells of elodea were more easily displaced by. Chloroplasts are the organelles in a plant cell that contain the chlorophyll plants use to convert light into sugars.
Cyclosis cytoplasmic streaming in plant cells elodea. Remember that plant cells contain both a cell membrane and a cell wall. In the case of elodea, filaments of actin molecules form a network of. In leaf cells of the higher aquatic plants, elodea and vallisneria, rotational stream ing of the cytoplasm is. It is found in both still and flowing waters, in lakes, ponds, pools, ditches, and quiet streams. What is the ground substance for viruses if they lack cytoplasm. Video clips are shown, with tips on viewing cyclosis with a student microscopeand links to a selection of web resources describing the phenomenom. It is very used in aquarium vegetation and also in artificial pounds. Cells online worksheet topic elodea plant elodea is a fresh water plant commonly used as vegetation in ponds and freshwater fish tanks. Cyclosis cytoplasmic streaming in plant cells elodea dic microscope 1250x. In the center of the cell is a large vacuole used to store water and other molecules. Cytoplasmic streaming in the higher plant elodea canadensis has been studied by light and electron microscopy. Cytoplasmic streaming, also called protoplasmic streaming and cyclosis, is the directed flow of cytosol and organelles around large fungal and plant cells. Aquarium hobbyist use elodea works well in many fish tanks.
Slide a slide b download this page, place in your notebook and answer the following questions. Diffusive motion of the cytoplasmic material was also apparent. Plant cells are much larger than animal cells and rely heavily on cytoplasmic streaming to distribute even small particles throughout cell. Elodea lab with cytoplasmic streaming and plasmolysis. The organelle that is correctly paired with its function is chloroplasts. Cytoplasmic streaming in elodea can be measured by observing the movement of.
The motion transports nutrients, proteins, and organelles within cells. Elodea is a genus of 6 species of aquatic plants often called the waterweeds described as a genus in 1803. Elodea canadensis is a species that works well for this activity, because it has thin, straight leaves. This movement aids in the delivery of organelles, nutrients, metabolites, genetic informati. Describe the shape and the location of chloroplasts. Elodea plants are native to the new world, though a number of species have established themselves as invasive. Increase the magnification to 100x and refocus the slide using the fine knob. Cytoplasmic streaming is defined as the movement of the fluid substance cytoplasm within a plant or animal cell. Cyclosis cytoplasmic streaming in plant cells elodea create. It provides space for the functioning of cell organelles. Heatlight slows down or stops cytoplasmic streaming, so minimize plates exposure to light.
Together with, or independent of, cytoplasmic streaming, different. Plant cell lab onion and elodea the biology corner. What are the causes of cytoplasmic streaming in elodea. Egeria densa, egeria brasiliensis is the general name given to these underwater weeds that belong to the group of the angiosperms. This is the case because the main part of the cell contains the central vacuole which pushes all the cytoplasm and organelles out toward the cell wall. As cytoplasm is a fluid, it acts as a buffer, protecting the cells genetic material and organelles from damage due to movement or collision with other cells.
Describe the locations of the nucleus and the majority of the chloroplasts in elodea cells. How does cytoplasmic streaming occur in elodea cells. View a prepared slide of elodea anacharis, which is an aquarium plant. Elodea definition of elodea by the free dictionary. What is clearly visible in plants cells which exhibit cytoplasmic streaming is the motion of the chloroplasts moving with the cytoplasmic flow. This is the mechanism for the transport of nutrients. The photographs show elodea leaves viewed under a light microscope at 200x slide a and 600x magnification slide b. The photochemical apparatus, its structure and function, brookhaven.
It is also often used in biology labs on cell structure because it forms nice, large cells that are easily observed under the microscope. Our observations began with making a simple wet mount of the leaf and viewing it under 100x and 400x magnification. Describe the functions of the components in elodea. This phenomenon is particularly prevalent in plant cells, often presenting strikingly regimented flow patterns. First discovered in the 1830s, the presence of cytoplasmic streaming helped convince biologists that.